Project

RNA-based therapy in Crohn’s disease

Identification and functional modulation of bacterial and human RNA-targets to prevent generation of pathogenic intestinal Th17cells: perspective for a novel RNA-based therapy in Crohn’s disease
Application Areas
Research Areas
Research Lines
Staff
WEBSITE

ITB Principal Investigator

Name

Identification and functional modulation of bacterial and human RNA-targets to prevent generation of pathogenic intestinal Th17cells: perspective for a novel RNA-based therapy in Crohn’s disease

Acronym

RNA-based therapy in Crohn’s disease

Location

Segrate

Start Date

2023

End Date

2026

Funder

MUR-PRIN2022

Partners

UniMI-IRCCS Cà Grande Policlinico di Milano (Dipartimenti di Fisiopatologia Medico-Chirurgica e dei Trapianti, Dipartimento di Bioscienze, Gastroenterologia, Malattie Infettive e Malattie Cutanee; Microbiologia)

This project represents a follow-up to Pathos-17, using microbiological, immunological, and transcriptomic approaches (Dual-RNASeq) on peripheral and intestinal dendritic cells (DCs), infected and uninfected by various bacterial mutants, derived from healthy individuals and Crohn’s disease patients. Aims include: 1) understanding microbiological, immunological, and molecular pathways, which regulate the persistence, in peripheral/intestinal DCs, of E. coli AIEC with the consequent activation of pathogenic Th1/17 cells; 2) characterizing the immune responses of E. coli AIEC-infected DCs in healthy individuals and Crohn’s patients; 3) functional validation of RNA targets, which promote AIEC-DC interaction/Th1/17 activation in the intestine; 4) formulation of preclinical models for the development of next-generation immunotherapies for Crohn’s disease.