Igea D’Agnano

The information published in this page are entirely managed by IGEA D'AGNANO who is completely responsible for it.

"Cell communication in cancer development"

Igea D'Agnano is PhD, expert in molecular and cellular experimental oncology, with high expertise in flow cytometry. 1986 Master Degree in Biological Sciences. 1991 Specialization in Clinical Pathology. Researcher CNR (1992-2005). Since 2006 Senior Scientist CNR. 1992 Visiting Scientist at Jefferson Cancer Institute, Philadelphia, USA. For many years, she focused her studies on new antitumor therapeutic strategies using synthetic molecules such as antisense oligonucleotides and mimetic oligopeptides directed towards the MYC oncogene. Since 2006 she has studied the role of the LMNA gene in the differentiation and progression of tumors of the nervous system. Since 2012 her research interests are focused on the study of circulating microRNAs and extracellular vesicles as mediators in cell-to-cell communication in order to identify biomarkers of early diagnosis in tumors. She is an expert in applying Flow Cytometry to the study of the cell cycle, apoptosis and extracellular vesicles. She served as member of the executive board of the Italian Society of Cytometry (2010-2016). She served as Associate Editor for the scientific journal Cell Death Discovery (CDD press/Nature). She serves as Associate Editor for the scientific journal Biomedicines (MDPI). Igea D'Agnano obtained various funding on projects focused on the study of tumor progression mechanisms aimed at identifying biomarkers and therapeutic targets, from CNR, MIUR, Ministry of Health. She has published over 60 scientific papers in peer-reviewed international journals.

Interest areas:

Experimental oncology - gene expression - cell-to-cell communication - microRNAs - extracellular vesicles


Contacts

Mobile: 3383591475
Phone: 02-26422 712
Skype: igea59
Address: Istituto di Tecnologie Biomediche Via Fratelli Cervi 93 20090 Segrate (MI)

International Ids


H-indexes

Google Scholar H-Index: 31
Scopus H-Index: 26
ISI-WoS H-Index: 26

Experience

Primo Ricercatore
Company: ITB - Istituto di tecnologie biomediche - Istituto di Tecnologie Biomediche (ITB)
Time period: 12/2018 - today

Primo Ricercatore
Company: CNR - Consiglio Nazionale delle Ricerche - Istituto di Biologia Cellulare e Neurobiologia (IBCN)
Time period: 01/2006 - 11/2018

Ricercatore
Company: CNR - Istituto Tecnologie Biomediche (ITB), Milano
Time period: 03/2002 - 12/2005

Ricercatore
Company: CNR - Consiglio Nazionale delle Ricerche - Istituto di Tecnologie BioMediche (ITBM), Roma
Time period: 12/1996 - 02/2002

Ricercatore a contratto a tempo determinato (ex art. 36, Legge 70/1975)
Company: CNR - Consiglio Nazionale delle Ricerche - Istituto di Tecnologie BioMediche, Roma
Time period: 03/1992 - 12/1996


Activities

Scientific projects

CLUSTER ALISEI, "CREATION OF A MULTIREGIONAL INFRASTRUCTURE FOR THE DEVELOPMENT OF ADVANCED THERAPIES FOR ORGAN AND TISSUE REGENERATION"
Related working experience: Primo Ricercatore
Role: Project Manager
Participation time period: 07/2017 - 08/2019

miRNome analysis and functional studies of cancer cell-released exosomes in experimental models of Glioblastoma (Interomics)
Related working experience: Primo Ricercatore
Role: Principal Investigator
Participation time period: 01/2015 - 07/2018
Involved organizations: ISTITUTO DI BIOLOGIA CELLULARE E NEUROBIOLOGIA - IBCN - SEDE MONTEROTONDO SCALO;

Extracellular microRNAs (miRNAs) have attracted great attention in cancer research since they are de-regulated in most types of cancers and can be potentially used as specific biomarkers and treatment-response predictors. Recently, microRNAs packed into tumour-released exosomes have been shown to contribute to tumor establishment and metastatic spread. Glioblastoma (GBM) is a very aggressive brain tumour with a very poor prognosis and no effective diagnostic or prognostic biomarkers are yet available. Our objective is to identify through "Omics" technologies a signature of miRNAs (miRNome) specifically expressed in exosomes released from GBM cell lines in vitro and from orthotopic human GBM in nude mice.


Optimisation of liver and intestine in vitro models for pharmacokinetics and pharmacodynamics studies
Role: Partecipante Unità Operativa
Participation time period: 10/2006 - 09/2010
Involved organizations: SI;

Studio degli effetti indotti dalla deregolazione del gene c-myc nei processi proliferativi e differenziativi di cellule umane
Role: Responsabile Unità Operativa
Participation time period: 11/1997 - 11/2000

Indicatori biologici di prognosi in tumori mammari ed applicazioni in oncologia clinica
Role: Responsabile Unità Operativa
Participation time period: 05/1995 - 05/1996
Involved organizations: ARMANDO FELSANI;

Circulating miRNAs induced by orthotopic human glioblastoma in mice
Role: Responsabile Unità Operativa

Sviluppo e validazione di strategie terapeutiche innovative indirizzate a contrastare meccanismi di sopravvivenza cellulare
Role: Responsabile Unità Operativa
Participation time period: 12/2002 -
Involved organizations: SI;

Teamworks, commissions or organisms

Società Italiana di Citometria
Role: membro del Consiglio Direttivo
Participation time period: 10/2010 - 10/2016

Teaching and scientific spreading

"Aggiornamenti e innovazioni della citometria nelle applicazioni cliniche e di ricerca e corsi satelliti di aggiornamento: la formazione continua del citometrista". Corso: "MicroRNA circolanti e vescicole extracellulari: analisi citometriche"
Related working experience: Primo Ricercatore
Location: Frascati
Role: Docente e coordinatore
Participation time period: 05/2019 - 05/2019

Applicazioni Innovative in Citometria
Location: Urbino
Role: Docente/coordinatore
Participation time period: 09/2014 - 09/2014

Applicazioni Innovative in Citometria
Location: Urbino
Role: Docente/coordinatore
Participation time period: 09/2012 - 09/2012

Apoptosi
Location: Università "La Tuscia", Viterbo
Role: Professore a contratto
Participation time period: 10/2010 - 12/2011

Chemioterapia Antitumorale
Location: Milano
Participation time period: 12/2006 - 04/2007
Involved organizations: UNIVERSITà DEGLI STUDI;

Chemioterapia Antitumorale
Location: Milano
Participation time period: 05/2006 - 11/2006
Involved organizations: UNIVERSITà DEGLI STUDI;

Chemioterapia antitumorale
Location: Milano
Participation time period: 11/2004 - 10/2005
Involved organizations: UNIVERSITà DEGLI STUDI;

citometria
Location: Università di Urbino
Participation time period: 10/2000 - 10/2000
Involved organizations: SCUOLA NAZIONALE DI CITOMETRIA GIC;

ciclo cellulare e apoptosi
Location: Direzione didattica Via Passaggi 2 Genova
Participation time period: 10/1996 - 10/1996
Involved organizations: SCUOLA INTERNAZIONALE DI ONCOLOGIA E MEDICINA SPERIMENTALE;

ciclo cellulare e apoptosi
Location: Direzione didattica Via Passaggi 2, Genova
Participation time period: 12/1994 - 12/1994
Involved organizations: SCUOLA INTERNAZIONALE DI ONCOLOGIA E MEDICINA SPERIMENTALE;

Citofluorimetria a flusso
Location: Segreteria organizzativa Via A.Passaggi 2, Genova
Participation time period: 11/1990 - 11/1992
Involved organizations: SCUOLA INTERNAZIONALE DI ONCOLOGIA E MEDICINA SPERIMENTALE;

Structures responsibility

INMM
Role: Responsabile


Education

Specialista in Patologia Clinica
Sapienza università di Roma
Graduation date: 1991-11-26
Thesis: Cambiamenti indotti dal solvente polare N-metilformamide sull'attività proliferativi e il pattern antigenico della linea cellulare di carcinoma del colon HT29

Scienze Biologiche
Sapienza Università di Roma
Graduation date: 1986-12-16
Thesis: Studio delle caratteristiche biologiche di linee autologhe di tumore polmonare umano: sensibilità ad agenti antitumorali


Languages

Production Understanding
Written Oral Written Oral Notes
English - Full professional proficiencyC1C1C1C1

Research activities classification (ERC)

GENETICS, GENOMICS, BIOINFORMATICS AND SYSTEMS BIOLOGY: GENETICS, POPULATION GENETICS, MOLECULAR GENETICS, GENOMICS, TRANSCRIPTOMICS, PROTEOMICS, METABOLOMICS, BIOINFORMATICS, COMPUTATIONAL BIOLOGY, BIOSTATISTICS, BIOLOGICAL MODELLING AND SIMULATION, SYSTEMS BIOLOGY, GENETIC EPIDEMIOLOGY
LIFE SCIENCES
MOLECULAR AND STRUCTURAL BIOLOGY AND BIOCHEMISTRY: MOLECULAR BIOLOGY, BIOCHEMISTRY, BIOPHYSICS, STRUCTURAL BIOLOGY, BIOCHEMISTRY OF SIGNAL TRANSDUCTION

Skills

BIOLOGIA MOLECOLARE: TECNICHE DI BASE PER L'ISOLAMENTO, LA PURIFICAZIONE E L'ANALISI DI ACIDI NUCLEICI E PROTEINE (ENDOGENE ED ESOGENE). TECNICHE PER LO STUDIO DI ESPRESSIONE GENICA, INTERAZIONE DNA-PROTEINE, INTERAZIONE PROTEINA-PROTEINA (NORTHERN BLOT, RNASI PROTECTION, RUN-ON, RT- QPCR, WESTERN BLOT, IMMUNOPRECIPITAZIONE E CO-IMMUNOPRECIPITAZIONE, PULSE-CHASE, EMSA, DNA AFFINITY CHROMATOGRAPHY BINDING ASSAY, CHROMATIN IMMUNOPRECIPITATION)

Language certifications

First Certificate in EnglishDate: 1982

Research products

Loredana Guglielmi 1,+, Marta Nardella 2,+, Carla Musa 3,+, Ingrid Cifola 1,+, Manuela Porru 4, Beatrice Cardinali 3, Ilaria Iannetti 3, Chiara Di Pietro 3, Giulia Bolasco 5, Valentina Palmieri 6,7, Laura Vilardo 1, Nicolò Panini 8, Fabrizio Bonaventura 3, Massimiliano Papi 6,7, Ferdinando Scavizzi 3, Marcello Raspa 3, Carlo Leonetti 4,?, Germana Falcone 3,?, Armando Felsani 9,? and Igea D'Agnano 1,*,?

Circulating miRNAs in Small Extracellular Vesicles Secreted by a Human Melanoma Xenograft in Mouse Brains

Loredana Guglielmi 1,+, Marta Nardella 2,+, Carla Musa 3,+, Ingrid Cifola 1,+, Manuela Porru 4, Beatrice Cardinali 3, Ilaria Iannetti 3, Chiara Di Pietro 3, Giulia Bolasco 5, Valentina Palmieri 6,7, Laura Vilardo 1, Nicolò Panini 8, Fabrizio Bonaventura 3, Massimiliano Papi 6,7, Ferdinando Scavizzi 3, Marcello Raspa 3, Carlo Leonetti 4,?, Germana Falcone 3,?, Armando Felsani 9,? (2020) in Cancers (Basel)
Parafioriti A.; Cifola I.; Gissi C.; Pinatel E.; Vilardo L.; Armiraglio E.; Di Bernardo A.; Daolio P.A.; Felsani A.; D'Agnano I.; Berardi A.C.

Expression profiling of microRNAs and isomiRs in conventional central chondrosarcoma

(2020) in Cell Death Discovery
D'Agnano I1, Berardi AC2.

Extracellular Vesicles, A Possible Theranostic Platform Strategy for Hepatocellular Carcinoma-An Overview

(2020) in Cancers (Basel)
Camerlingo R1, Miceli R2, Marra L1, Rea G3, D'Agnano I4,5, Nardella M6, Montella R7, Morabito A8, Normanno N1, Tirino V7, Rocco G9

Conditioned medium of primary lung cancer cells induces EMT in A549 lung cancer cell line by TGF-ß1 and miRNA21 cooperation

(2019) in PloS one
Igea D'Agnano 1 and Anna Concetta Berardi 2

Extracellular Vesicles, A Possible Theranostic Platform Strategy for Hepatocellular Carcinoma

(2019) in Cancers (Basel)
Berardocco, Martina; Radeghieri, Annalisa; Busatto, Sara; Gallorini, Marialucia; Raggi, Chiara; Gissi, Clarissa; D'Agnano, Igea; Bergese, Paolo; Felsani, Armando; Berardi, Anna C.

RNA-seq reveals distinctive RNA profiles of small extracellular vesicles from different human liver cancer cell lines

(2017) in Oncotarget
Guglielmi L1, Nardella M1, Musa C1, Iannetti I1, Arisi I2, D'Onofrio M2, Storti A2, Valentini A3, Cacci E4, Biagioni S4, Augusti-Tocco G4, D'Agnano I5, Felsani A6

Lamin A/C Is Required for ChAT-Dependent Neuroblastoma Differentiation

(2016) in Molecular neurobiology (Online)
Marta Nardella1, Loredana Guglielmi1, Carla Musa1, Ilaria Iannetti1, Giovanna Maresca1, Donatella Amendola2, Manuela Porru3, Elisabetta Carico4, Giuseppe Sessa5, Rosalba Camerlingo5, Carlo Dominici6,7, Francesca Megiorni6, Marika Milan1, Claudia Bearzi1,8, Roberto Rizzi1,8, Giuseppe Pirozzi5, Carlo Leonetti3, Barbara Bucci2, Delio Mercanti1, Armando Felsani1, Igea D'Agnano1

Down-regulation of the Lamin A/C in neuroblastoma triggers the expansion of tumor initiating cells

Marta Nardella1, Loredana Guglielmi1, Carla Musa1, Ilaria Iannetti1, Giovanna Maresca1, Donatella Amendola2, Manuela Porru3, Elisabetta Carico4, Giuseppe Sessa5, Rosalba Camerlingo5, Carlo Dominici6,7, Francesca Megiorni6, Marika Milan1, Claudia Bearzi1,8, Roberto Rizzi1,8, Giuseppe Pirozzi5, Carlo Leonetti3, (2015) in Oncotarget
Germana Falcone, Armando Felsani and Igea D'Agnano

Signaling by exosomal microRNAs in cancer

(2015) in Journal of experimental & clinical cancer research (Online)
Amendola D.; Nardella M.; Guglielmi L.; Cerquetti L.; Carico E.; Alesi V.; Porru M.; Leonetti C.; Bearzi C.; Rizzi R.; D'Agnano I.; Stigliano A.; Novelli G.; Bucci B.

Human placenta-derived neurospheres are susceptible to transformation after extensive in vitro expansion

(2014) in Stem cell research & therapy
Visualize all the publications (since 2016)