Ingrid Cifola

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My research activity focuses on human cancer genomics and application of high-throughput technologies, such as next-generation sequencing (NGS) and microarray platforms, to perform "-omics" analyses for translational medicine purposes. Working in close cooperation with hospital and university research groups, I used such technologies to perform genomic, transcriptomic and epigenetic analyses in several human cancer pathologies, among which renal cell carcinoma, cutaneous malignant melanoma, acute myeloid leukemia, acute lymphoblastic leukemia, myeloproliferative neoplasms and multiple myeloma. I used high-density microarray platforms to identify chromosomal aberrations, to analyse gene expression profiles and to study promoter methylation. Then, I applied NGS technologies for whole-exome sequencing (WES) or gene target sequencing to identify mutations and/or structural genomic rearrangements, for transcriptomic profiling and identification of particular transcripts, such as fusion transcripts, conjoined genes and circular RNAs (RNA-seq), for miRNA expression profiling in extracellular vesicles released by cancer cells (small RNA-seq), for single-cell transcriptomics analysis of tumor samples (single-cell RNA-seq). Moreover, I applied integrated approaches to combine different kind of omics data, e.g. genomic profile and gene expression data, aiming to obtain a more complete view of molecular mechanisms underlying tumorigenesis and to identify candidate crucial genes for tumor biology. The final goal of my studies is to contribute to improve the knowledge of the molecular mechanisms involved in malignant transformation and progression and to help the identification of novel candidate markers useful for early tumor diagnosis or prognostic prediction, or as molecular targets for new therapeutic strategies. A particular interest is addressed to "liquid biopsy" approaches for a non-invasive and early tumor diagnosis and post-therapy patient's monitoring. These are the basis to realize a "personalized medicine" that maximizes therapy efficacy and minimizes toxic side effects, thus improving life expectation and quality of cancer patients. I have been involved in many research projects funded by Italian MIUR, Cariplo and AIRC Foundations. My scientific activity is documented by 35 papers on peer-reviewed international journals (Scopus h 19).

Interest areas:

Application of high-throughput technologies for molecular characterization of human cancer pathologies, among which renal cell carcinoma (RCC), cutaneous malignant melanoma, acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), myeloproliferative neoplasms (MPN), multiple myeloma (MM). Application of high-density microarray platforms for human DNA copy number profiling, gene and miRNA expression analysis, and promoter methylation analysis. Application of next-generation sequencing (NGS) for whole-exome or target sequencing and identification of single nucleotide variants (SNV) and copy number variants (CNV), for gene expression profiling and identification of fusion transcripts, conjoined genes and circular RNAs (RNA-seq), for miRNA expression profiling in extracellular vesicles released by cancer cells (small RNA-seq), for single-cell transcriptomics analysis of tumor samples (single-cell RNA-seq).


Contacts

Phone: +39-02-26422705
Address: Istituto di Tecnologie Biomediche (ITB), CNR, via F.lli Cervi 93, 20090 Segrate (MI)
Pec: ingrid.cifola@pec.it

International Ids

Scopus Author ID: 8312798100

H-indexes

Google Scholar H-Index: 21
Scopus H-Index: 19

Curriculum Vitae


Research products

Tolomeo, Doron; Agostini, Antonio; Macchia, Gemma; L'Abbate, Alberto; Severgnini, Marco; Cifola, Ingrid; Frassanito, Maria Antonia; Racanelli, Vito; Solimando, Antonio Giovanni; Haglund, Felix; Mertens, Fredrik; Storlazzi, Clelia Tiziana

BL1391: an established cell line from a human malignant peripheral nerve sheath tumor with unique genomic features

(2021) in Human cell
Parafioriti A.; Cifola I.; Gissi C.; Pinatel E.; Vilardo L.; Armiraglio E.; Di Bernardo A.; Daolio P.A.; Felsani A.; D'Agnano I.; Berardi A.C.

Expression profiling of microRNAs and isomiRs in conventional central chondrosarcoma

(2020) in Cell Death Discovery
Loredana Guglielmi 1,+, Marta Nardella 2,+, Carla Musa 3,+, Ingrid Cifola 1,+, Manuela Porru 4, Beatrice Cardinali 3, Ilaria Iannetti 3, Chiara Di Pietro 3, Giulia Bolasco 5, Valentina Palmieri 6,7, Laura Vilardo 1, Nicolò Panini 8, Fabrizio Bonaventura 3, Massimiliano Papi 6,7, Ferdinando Scavizzi 3, Marcello Raspa 3, Carlo Leonetti 4,?, Germana Falcone 3,?, Armando Felsani 9,? and Igea D'Agnano 1,*,?

Circulating miRNAs in Small Extracellular Vesicles Secreted by a Human Melanoma Xenograft in Mouse Brains

Loredana Guglielmi 1,+, Marta Nardella 2,+, Carla Musa 3,+, Ingrid Cifola 1,+, Manuela Porru 4, Beatrice Cardinali 3, Ilaria Iannetti 3, Chiara Di Pietro 3, Giulia Bolasco 5, Valentina Palmieri 6,7, Laura Vilardo 1, Nicolò Panini 8, Fabrizio Bonaventura 3, Massimiliano Papi 6,7, Ferdinando Scavizzi 3, Marcello Raspa 3, Carlo Leonetti 4,?, Germana Falcone 3,?, Armando Felsani 9,? (2020) in Cancers (Basel)
Daniela Gaglio 1,2, Marcella Bonanomi 2,3, Silvia Valtorta 1,2,4, Rohit Bharat 23, Marilena Ripamonti 1,2, Federica Conte 2,5, Giulia Fiscon 2,5, Nicole Righi 2,3, Elisabetta Napodano 1,2, Federico Papa 2,5, Isabella Raccagni 1,2,6, Seth J Parker 7,8, Ingrid Cifola 9, Tania Camboni 9, Paola Paci 2,5,10, Anna Maria Colangelo 2,3, Marco Vanoni 2,3, Christian M Metallo 7,8, Rosa Maria Moresco 1,2,4, Lilia Alberghina 2,3

Disruption of redox homeostasis for combinatorial drug efficacy in K-Ras tumors as revealed by metabolic connectivity profiling

(2020) in Cancer & metabolism
Bombelli, Silvia; Torsello, Barbara; De Marco, Sofia; Lucarelli, Giuseppe; Cifola, Ingrid; Grasselli, Chiara; Strada, Guido; Bovo, Giorgio; Perego, Roberto A.; Bianchi, Cristina

36-kDa Annexin A3 Isoform Negatively Modulates Lipid Storage in Clear Cell Renal Cell Carcinoma Cells

(2020) in The American journal of pathology (Print)
Cupaioli FA, Di Nanni N, Pelucchi P, Cifola I, Villa L, Raggi ME, Milanesi L, Mosca M, Mezzelani A

Linked read sequencing reveals new genetic variants in autism

(2020) Neurogenetics, Nature Conferences, Virtual (Covid19), 29/07/2020
Tirone, Mario; Giovenzana, Anna; Vallone, Arianna; Zordan, Paola; Sormani, Martina; Nicolosi, Pier Andrea; Meneveri, Raffaela; Gigliotti, Carmen Rosaria; Spinelli, Antonello E.; Bocciardi, Renata; Ravazzolo, Roberto; Cifola, Ingrid; Brunelli, Silvia

Severe Heterotopic Ossification in the Skeletal Muscle and Endothelial Cells Recruitment to Chondrogenesis Are Enhanced by Monocyte/Macrophage Depletion

(2019) in Frontiers in immunology
Gemma Macchia, Marco Severgnini, Stefania Purgato, Doron Tolomeo, Hilen Casciaro, Ingrid Cifola, Alberto L'Abbate, Anna Loverro, Orazio Palumbo, Massimo Carella, Laurence Bianchini, Giovanni Perini, Gianluca De Bellis, Fredrik Mertens, Mariano Rocchi and Clelia T. Storlazzi

The Hidden Genomic and Transcriptomic Plasticity of Giant Marker Chromosomes in Cancer

(2018) in Genetics (Austin Tex., Online)
Cindy Buffone1, Alicia Martinez-Lopez1, Thomas Fricke1, Silvana Opp1, Marco Severgnini2, Ingrid Cifola2, Luca Petiti2, Stella Frabetti3, Katarzyna Skorupka4, Kaneil K. Zadrozny4, Barbie K. Ganser-Pornillos4, Owen Pornillos4, Francesca Di Nunzio3+ and Felipe Diaz-Griffero1+

Nup153 Unlocks the Nuclear Pore Complex for HIV-1 Nuclear Translocation in Non-dividing Cells

(2018) in Journal of virology (Online)
Romano, Oriana; Cifola, Ingrid; Poletti, Valentina; Severgnini, Marco; Peano, Clelia; De Bellis, Gianluca; Mavilio, Fulvio; Miccio, Annarita

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions.

(2017) in Journal of visualized experiments
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